Journal: Stem Cells International

Article Title: Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells

doi: 10.1155/2016/1406304

Figure Lengend Snippet: Interaction of LO-EPC with activated HAEC. Surface expression of adhesion molecules in HAEC after stimulation with 0.05 ng/mL TNF α (a). Surface expressions of E-selectin, ICAM-1, and VCAM-1 were quantified using flow cytometry. The graph shows relative Mean Fluorescence Intensity (MFI) normalised to untreated cells and represents the mean ± SE of three experiments. ∗∗ p < 0.01. Activation of HAEC did not increase the interaction with LO-EPC (b). 4 × 10 5 LO-EPC were perfused to 3 × 10 4 HAEC for 4 min and the data represented as mean ± SE of three experiments.

Article Snippet: To study TNF α mediated HAEC activation, control HAEC and HAEC treated with 0.05 ng/ μ L TNF α (R&D System, UK), for 4 hours at 37°C, were collected in 100 μ L 1% bovine serum albumin (BSA, Sigma) in PBS and incubated with fluorescein conjugated anti-human E-selectin antibodies (R&D System), APC conjugated anti-human ICAM-1 (BD), and PE/Cy5 conjugated anti-human VCAM-1 (Bio Legend, UK), together with the respective isotype control antibodies.

Techniques: Expressing, Flow Cytometry, Fluorescence, Activation Assay